{"product_id":"biolegend-302630","title":"Biolegend, 302630, Brilliant Violet 421™ anti-human CD25 Antibody, 100tests","description":"\u003cp\u003eCD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes\/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunocytochemistry3.\u003cbr\u003e\nAdditional Product Notes: View more applications data for this product in our Scientific Poster Library.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed\u003cbr\u003e\nProduct Citations: Zhang C, et al. 2023. Front Immunol. 14:1182409. PubMed Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed Ringelstein-Harlev S, et al. 2023. Eur J Haematol. 110:371. PubMed Hsiao CC, et al. 2023. iScience. 26:105785. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Shoda H, et al. 2017. Arthritis Research \u0026amp; Therapy . 10.1186\/s13075-017-1308-y. PubMed Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed Nesspor TC, et al. 2020. Sci Rep. 10:7557. PubMed Huang C, et al. 2020. Theranostics. 10:10498. PubMed Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed Lam AJ, et al. 2021. Cell Reports. 36(5):109494. PubMed Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed Yang L, et al. 2020. Genes Dis. 7:128. PubMed Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed Nowak A, et al. 2018. Front Immunol. 0.513194444. PubMed Nakachi S, et al. 2017. Arthritis Research \u0026amp; Therapy . 10.1186\/s13075-017-1309-x. PubMed Gao X, et al. 2022. iScience. 25:104911. PubMed Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed Paul S, et al. 2021. Sci Transl Med. 13:. PubMed Hernndez DC, et al. 2021. Immunity. :. PubMed Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed Pandya J, et al. 2016. J Leukoc Biol. 100: 823 - 833. PubMed Manuszak C, et al. 2020. J Biol Methods. 7:e131. PubMed Li H, et al. 2020. Front Oncol. 1.477083333. PubMed Nowatzky J, et al. 2020. J Vis Exp. . PubMed Fu J, et al. 2021. J Clin Invest. 131:. PubMed Lin-Shiao E, et al. 2022. Nucleic Acids Res. 50:1256. PubMed\u003cbr\u003e\nRRID: AB_10896914 (BioLegend Cat. No. 302629) AB_11126749 (BioLegend Cat. No. 302630)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, 55 kD\u003cbr\u003e\nDistribution: Activated T cells and B cells, monocytes\/macrophages, Treg\u003cbr\u003e\nFunction: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex\u003cbr\u003e\nLigand\/Receptor: IL-2\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.\u003cbr\u003e\nGene ID: 3559\u003cbr\u003e\nUniProt: View information about CD25 on UniProt.org\u003cbr\u003e\nClone: BC96\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V T-072\u003cbr\u003e\nOther Names: Low affinity IL-2R, IL-2R α chain, Tac, p55\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862904295593,"sku":"302630","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-302630","provider":"Iright","version":"1.0","type":"link"}