{"product_id":"biolegend-303402","title":"Biolegend, 303402, Purified anti-human CD33 Antibody, 100μg","description":"\u003cp\u003eCD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate\/lectin binding activity.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human myeloid leukaemia cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF® - Verified ICC, IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 \u0026amp; 303438).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)\u003cbr\u003e\nProduct Citations: Consonni FM, et al. 2023. Front Immunol. 14:1168455. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed Wen J, et al. 2020. J Pathol Clin Res. 0.36875. PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed Henrick BM, et al. 2021. Cell. . PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Hou Z, et al. 2020. Oncol Lett. 19:3982. PubMed Crawford LB, et al. 2021. J Virol. 95:. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed\u003cbr\u003e\nRRID: AB_314346 (BioLegend Cat. No. 303402)\u003cbr\u003e\nStructure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD\u003cbr\u003e\nDistribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells\u003cbr\u003e\nFunction: Adhesion and lectin activity\u003cbr\u003e\nLigand\/Receptor: Sugar chains containing sialic acid\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005.\u003cbr\u003e\nGene ID: 945\u003cbr\u003e\nUniProt: View information about CD33 on UniProt.org\u003cbr\u003e\nClone: WM53\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: IV M-505\u003cbr\u003e\nOther Names: Siglec-3, gp67, p67\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864094527657,"sku":"303402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-303402","provider":"Iright","version":"1.0","type":"link"}