{"product_id":"biolegend-304331","title":"Biolegend, 304331, Brilliant Violet 711™ anti-human CD49d Antibody, 25tests","description":"\u003cp\u003eCD49d is a 150 kD α integrin chain known as α 4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α 4 β 1 , VLA-4) or β7 (α 4 β 7 ). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α 4 β 7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3 + cells.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cat, Cow, Chimpanzee, Common Marmoset, Dog, Horse, Sheep, Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710\/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation2,3. The Ultra-LEAF™ Purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304339 and 304340).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim) Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed Palacious F, et al. 2010. Blood 115:4488. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Mattapallil MJ, et al. 2011. J. Immunol. 187:1977. PubMed\u003cbr\u003e\nProduct Citations: Campisi L, et al. 2022. Nature. 606:945. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Metzemaekers M, et al. 2021. Clin Transl Immunology. 10:e1271. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed\u003cbr\u003e\nRRID: AB_2687197 (BioLegend Cat. No. 304331) AB_2687198 (BioLegend Cat. No. 304332)\u003cbr\u003e\nStructure: Integrin, type I transmembrane glycoprotein, 150 kD.\u003cbr\u003e\nDistribution: T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.\u003cbr\u003e\nFunction: Lymphocyte migration, T cell activation, stem cell differentiation.\u003cbr\u003e\nLigand\/Receptor: Fibronectin, VCAM-1, MAdCAM-1\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4). 2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.\u003cbr\u003e\nGene ID: 3676\u003cbr\u003e\nUniProt: View information about CD49d on UniProt.org\u003cbr\u003e\nClone: 9F10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V S215\u003cbr\u003e\nOther Names: VLA-4 α chain, α4 integrin, Integrin α4 chain, ITGA4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862513668265,"sku":"304331","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-304331","provider":"Iright","version":"1.0","type":"link"}