{"product_id":"biolegend-306012","title":"Biolegend, 306012, APC anti-human CD123 Antibody, 100tests","description":"\u003cp\u003eCD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human IL-3Rα transfected COS cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sun Q, et al. 1996. Blood 87:83. (IP, WB) Herling M, et al. 2003. Blood 101:5007. (IHC) Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)\u003cbr\u003e\nProduct Citations: Zhang X, et al. 2022. Commun Biol. 5:1332. PubMed Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed Kitai H, et al. 2022. BMC Biol. 20:248. PubMed Vijayakumar B, et al. 2022. Immunity. . PubMed González-Tajuelo R, et al. 2020. Front Immunol. 11:588212. PubMed Oliveira JAP, et al. 2021. J Leukoc Biol. 110:167. PubMed Ugawa M, et al. 2021. Elife. 10:. PubMed Thompson EA, et al. 2021. Cell Rep. 108863:34. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed Lu J, et al. 2021. Front Immunol. 12:732612. PubMed Georgakis S, et al. 2021. JCI Insight. 6:. PubMed Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed Pierog PL, et al. 2018. J Immunol. 200:186. PubMed Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed Labidi-Galy S, et al. 2011. Cancer Res. 71:5423. PubMed Shevchenko JA, et al. 2020. Oncol Rep. 43:671. PubMed Conrad C, et al. 2018. Nat Commun. 9:25. PubMed\u003cbr\u003e\nRRID: AB_439778 (BioLegend Cat. No. 306011) AB_439779 (BioLegend Cat. No. 306012)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD\u003cbr\u003e\nDistribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes\u003cbr\u003e\nFunction: Hematopoietic cell proliferation, differentiation\u003cbr\u003e\nLigand\/Receptor: IL-3\u003cbr\u003e\nCell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.\u003cbr\u003e\nGene ID: 3563\u003cbr\u003e\nUniProt: View information about CD123 on UniProt.org\u003cbr\u003e\nClone: 6H6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-3Rα, IL-3 Receptor alpha\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862480441513,"sku":"306012","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-306012","provider":"Iright","version":"1.0","type":"link"}