{"product_id":"biolegend-306516","title":"Biolegend, 306516, PerCP\/Cyanine5.5 anti-human CD184 (CXCR4) Antibody, 100tests","description":"\u003cp\u003eCD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes\/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Chimpanzee, Sooty Mangabey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CP-MAC-infected Sup-T1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 \u0026amp; 306540).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): McKnight A, et al. 1997. J. Virol. 71:1692. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF) Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC) Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block) Ullrich CK, et al. 2000. Blood 96:1438. (Block) Murga M, et al. 2005. Blood 105:1992. (IF) Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed Isnardi I, et al. 2010. Blood 115:5026. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Fischer T, et al. 2008. PLoS One 3:e4069. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)\u003cbr\u003e\nProduct Citations: Tang WWC, et al. 2022. Nat Cell Biol. 24:448. PubMed Bellini N, et al. 2022. iScience. 25:105234. PubMed Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed Quoyer J, et al. 2013. Proc Natl Acad Sci U S A. 110:5088. PubMed Paradis J, et al. 2015. Proc Natl Acad Sci U S A. 112: 5160-5168. PubMed Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed\u003cbr\u003e\nRRID: AB_10639734 (BioLegend Cat. No. 306515) AB_10642818 (BioLegend Cat. No. 306516)\u003cbr\u003e\nStructure: Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD\u003cbr\u003e\nDistribution: T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells\u003cbr\u003e\nFunction: B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development\u003cbr\u003e\nLigand\/Receptor: SDF-1 receptor, coreceptor for X4 HIV-1\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657. 2. Loetscher P, et al. 2000. Adv. Immunol. 74:127. 3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.\u003cbr\u003e\nGene ID: 7852\u003cbr\u003e\nUniProt: View information about CD184 on UniProt.org\u003cbr\u003e\nClone: 12G5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VII 70204\u003cbr\u003e\nOther Names: CXCR4, Fusin\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860797739177,"sku":"306516","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-306516","provider":"Iright","version":"1.0","type":"link"}