{"product_id":"biolegend-307301","title":"Biolegend, 307301, Purified anti-human CD262 (DR5, TRAIL-R2) Antibody, 25μg","description":"\u003cp\u003eDR5 is 55 kD member 10B of the TNF receptor superfamily (TNFRSF10B), also known as TRAIL-R2, TRICK2, KILLER, and CD262. It binds the cytotoxic ligand TRAIL and induces apoptosis. The DR5 receptor is broadly expressed on a variety of normal tissues and many tumors. DR5 expression has been reported to be upregulated in human cells by interferon-α, 2-methoxyestradiol, and paclitaxel, and downregulated by adenoviral E3 proteins.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Extracellular domain of DR5-human IgG1 Fc fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For successful immunofluorescent staining results, it may be important to maximize signal over background by using a high sensitivity, three-layer staining technique (e.g., biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Uno K, et al. 2003. Blood 101:3658. Sato K, et al. 2005. J. Immunol. 174:4025. Shi J, et al. 2008. Blood 111:1309.PubMed Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed\u003cbr\u003e\nProduct Citations: Vunnam N, et al. 2020. Biochemistry. 59:3856. PubMed Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed Winterberg D, et al. 2021. J Clin Med. 10:. PubMed Mitchell M, et al. 2017. Nat Commun. 10.1038\/ncomms14179. PubMed Shi J, et al. 2008. Blood. 111:1309. PubMed\u003cbr\u003e\nRRID: AB_314672 (BioLegend Cat. No. 307302)\u003cbr\u003e\nStructure: TNFR superfamily, 55 kD\u003cbr\u003e\nDistribution: Broadly expressed on normal and tumor cells\u003cbr\u003e\nFunction: Induces apoptosis\u003cbr\u003e\nLigand\/Receptor: TRAIL\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. MacFarlane M, et al. 1997. J. Biol. Chem. 272:25417. 2. Walczak H, et al. 1997. EMBO J. 16:5386. 3. Shigeno M, et al. 2003. Oncogene 22:1653. 4. LaVallee T, et al. 2003. Cancer Res. 63:468. 5. Nimmanapalli R, et al. 2001. Cancer Res. 61:759\u003cbr\u003e\nGene ID: 8795\u003cbr\u003e\nUniProt: View information about CD262 on UniProt.org\u003cbr\u003e\nClone: DJR2-2 (2-6)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TRAIL-R2, KILLER, TRICK2, TNFRSF10B, Ly98, CD262\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863011446953,"sku":"307301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-307301","provider":"Iright","version":"1.0","type":"link"}