{"product_id":"biolegend-309404","title":"Biolegend, 309404, PE anti-human CD275 (B7-H2, ICOSL) Antibody, 100tests","description":"\u003cp\u003eB7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes\/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human B7H2-mIg fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563.\u003cbr\u003e\nProduct Citations: Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Schatton T, et al. 2010. Cancer Res. 70:697. PubMed Dianzani C, et al. 2010. J Immunol. 185:3970. PubMed O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed Siedlik J, et al. 2017. J Immunol Methods. 10.4049\/jimmunol.1700003. PubMed\u003cbr\u003e\nRRID: AB_314769 (BioLegend Cat. No. 309403) AB_314770 (BioLegend Cat. No. 309404)\u003cbr\u003e\nStructure: Ig superfamily, B7 family, 40 kD\u003cbr\u003e\nDistribution: B cells and dendritic cells\u003cbr\u003e\nFunction: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production\u003cbr\u003e\nLigand\/Receptor: ICOS\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Wang S, et al. 2002. J. Biol. Chem. 96:2808. Wong SC, et al. 2003. Blood 102:1831.\u003cbr\u003e\nGene ID: 23308\u003cbr\u003e\nUniProt: View information about CD275 on UniProt.org\u003cbr\u003e\nClone: 2D3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862317191337,"sku":"309404","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-309404","provider":"Iright","version":"1.0","type":"link"}