{"product_id":"biolegend-310205","title":"Biolegend, 310205, FITC anti-human CD45RB Antibody, 25tests","description":"\u003cp\u003eCD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes\/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human thymocytes and T lymphocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The MEM-55 antibody is specific for a glycosylated-CD45RB epitope that is highly expressed on memory B cells and plasmablasts but not on naïve B cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Horejsi V, et al. 1988. Folia Biol. 34:23. Bazil V, et al. 1989. Immunogenetics 29:202. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Koethe S, et al. 2011. J. Leukoc. Biol. 90:5.\u003cbr\u003e\nProduct Citations: Koers J, et al. 2022. Eur J Immunol. 52:1630. PubMed Kaneko N, et al. 2020. Cell. 183(1):143-157.e13. PubMed Koers J, et al. 2022. Front Immunol. 13:891316. PubMed Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed\u003cbr\u003e\nRRID: AB_2687313 (BioLegend Cat. No. 310205) AB_2687314 (BioLegend Cat. No. 310206)\u003cbr\u003e\nStructure: Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)\u003cbr\u003e\nDistribution: Thymocytes, T cell subset, B cells, monocytes\/macrophages, granulocytes, dendritic cells\u003cbr\u003e\nFunction: Enhances TCR and BCR signaling\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.\u003cbr\u003e\nGene ID: 5788\u003cbr\u003e\nUniProt: View information about CD45RB on UniProt.org\u003cbr\u003e\nClone: MEM-55\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V CD45.08\u003cbr\u003e\nOther Names: CD45RB\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866464407721,"sku":"310205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-310205","provider":"Iright","version":"1.0","type":"link"}