{"product_id":"biolegend-310403","title":"Biolegend, 310403, PE anti-human CD129 (IL-9 R) Antibody, 25tests","description":"\u003cp\u003eCD129 is known as the 57 kD IL-9 receptor. It is a member of the hematopoietin receptor superfamily. Although the α-chain of the receptor binds IL-9 with high affinity, interaction with the γ-chain (CD132) of the IL-2 receptor is required for signaling. The IL-9 receptor is expressed at low levels on eosinophils, mast cells, macrophages, B lymphocytes, T lymphocytes, and erythroid progenitors. IL-9 receptor binding initiates STAT activation required for the proliferative and anti-apoptotic effects of this cytokine. In humans, signals from the IL-9 receptor appear to be critical for intrathymic T cell development. IL-9 binding has been shown to increase IL-5 receptor expression and promote survival in human eosinophils.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: inhibition the binding of IL-9 to the high affinity a-chain of the human IL-9 receptor1,2, and ELISA for detection of soluble IL-9R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 310404) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310408).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): De Smedt M, et al. 2000. J. Immunol. 164:1761. Pilette C, et al. 2002. J. Immunol. 168:4103. Demoulin JB, et al.1996. Mol. Cell. Biol. 16 (9):4710.\u003cbr\u003e\nProduct Citations: Das S, et al. 2021. J Immunol. 206:2740. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Bal S, et al. 2016. Nat Immunol. 10.1038\/ni.3444. PubMed Xiao M, et al. 2017. Blood. 129:3196. PubMed\u003cbr\u003e\nRRID: AB_314816 (BioLegend Cat. No. 310403) AB_314817 (BioLegend Cat. No. 310404)\u003cbr\u003e\nStructure: Cytokine receptor family, type I membrane protein or soluble, associate with common γ chain(CD132), 57 kD\u003cbr\u003e\nDistribution: Eosinophils, mast, thymocytes, erythroid, myeloid progenitors, macrophages\u003cbr\u003e\nFunction: Activates STAT; critical for intrathymic T cell development\u003cbr\u003e\nLigand\/Receptor: IL-9\u003cbr\u003e\nCell Type: Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Demoulin JB, et al.1996. Mol. Cell. Biol. 16:4710.\u003cbr\u003e\nGene ID: 3581\u003cbr\u003e\nUniProt: View information about CD129 on UniProt.org\u003cbr\u003e\nClone: AH9R7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD129, IL-9R\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862537326761,"sku":"310403","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-310403","provider":"Iright","version":"1.0","type":"link"}