{"product_id":"biolegend-311203","title":"Biolegend, 311203, Purified anti-human CD227 (MUC-1) Antibody, 100μg","description":"\u003cp\u003eMucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1\/SOS1\/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2\/neu. Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1\/SOS1\/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2\/neu.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Membrane enriched fractions of human metastatic breast carcinoma\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone DF3 recognizes the extracellular domain of the MUC1 protein, specifically the tandem repeat region.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hamada T, et al.2004. J Clin Pathol. 57:813-21 Hamada T, et al. 2012. Cancer. 118:5251-64. Yasui S, et al. 1994. Acta dermato-venereologica. 74:98-100.\u003cbr\u003e\nRRID: AB_3698967 (BioLegend Cat. No. 311203)\u003cbr\u003e\nStructure: 500-1000 kD proteoglycan (glycosylated form)\u003cbr\u003e\nDistribution: Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers\u003cbr\u003e\nInteraction: Complexes with SOS1 and Grb2 in RAS signaling\u003cbr\u003e\nLigand\/Receptor: ICAM-1 and Her2\/neu (ErbB2)\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Yasui S, et al. 1994. Acta Derm Venereol. 74:98-100. Ichige K, et al. 1995. Clin Cancer Res. 1:565-71.\u003cbr\u003e\nGene ID: 4582\u003cbr\u003e\nUniProt: View information about CD227 on UniProt.org\u003cbr\u003e\nClone: DF3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Mucin 1, Episialin, MAM-6, CA 15-3, PEM, CD227, EMA\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863007318185,"sku":"311203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-311203","provider":"Iright","version":"1.0","type":"link"}