{"product_id":"biolegend-311508","title":"Biolegend, 311508, Brilliant Violet 421™ anti-human CD137L (4-1BB Ligand) Antibody, 100tests","description":"\u003cp\u003e4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gullo C, et al. 2010. PLoS One. 5:e10845. (FC) PubMed\u003cbr\u003e\nProduct Citations: Kim GB, et al. 2022. Sci Transl Med. 14:eabo6135. PubMed Hader M, et al. 2020. Cancers (Basel). :12. PubMed\u003cbr\u003e\nRRID: AB_2687315 (BioLegend Cat. No. 311507) AB_2687316 (BioLegend Cat. No. 311508)\u003cbr\u003e\nStructure: TNF superfamily, 97 kD\u003cbr\u003e\nDistribution: Antigen presenting cells, activated B cells and T cells\u003cbr\u003e\nFunction: T cell proliferation, cytokine production\u003cbr\u003e\nLigand\/Receptor: 4-1BB (CDw137)\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Akiba H, et al. 2000. J. Exp. Med. 191:375. 2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488. 3. DeBenedette MA, et al. 1997. J. Immunol. 158:551. 4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.\u003cbr\u003e\nGene ID: 8744\u003cbr\u003e\nUniProt: View information about CD137L on UniProt.org\u003cbr\u003e\nClone: 5F4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 4-1BBL, CD137L, TNFSF9, 41BBL\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862461927593,"sku":"311508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-311508","provider":"Iright","version":"1.0","type":"link"}