{"product_id":"biolegend-311608","title":"Biolegend, 311608, PE\/Cyanine5 anti-human CD357 (GITR) Antibody, 100tests","description":"\u003cp\u003eGITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 311606).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kwon B, et al. 1999. J. Biol. Chem. 274:6056. Zhou Z, et al. 2008. Proc Natl Acad Sci USA . 105:5465 PubMed\u003cbr\u003e\nProduct Citations: Den Braanker H, et al. 2021. Front Immunol. 12:768113. PubMed\u003cbr\u003e\nRRID: AB_2240646 (BioLegend Cat. No. 311608)\u003cbr\u003e\nStructure: TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD\u003cbr\u003e\nDistribution: Activated lymphocytes\u003cbr\u003e\nFunction: Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2\/NIK pathway\u003cbr\u003e\nLigand\/Receptor: GITRL\u003cbr\u003e\nCell Type: Lymphocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.\u003cbr\u003e\nGene ID: 8784\u003cbr\u003e\nUniProt: View information about CD357 on UniProt.org\u003cbr\u003e\nClone: 621\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR, TNFRSF18\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: I see dim staining with anti human CD357 (GITR) clone 621 in blood and PBMCs.\u003cbr\u003e\nA: Our testing has found that anti human CD357 (GITR) clone 621 stains dim with blood and PBMCs and may only be suitable for situations where GITR levels are high, i.e. stimulated cells or specific cell lines expressing high levels of GITR.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862466220201,"sku":"311608","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-311608","provider":"Iright","version":"1.0","type":"link"}