{"product_id":"biolegend-312612","title":"Biolegend, 312612, APC anti-human CD158b\/j (KIR2DL2\/L3\/S2) Antibody, 100tests","description":"\u003cp\u003eCD158b is expressed on natural killer cells and a subset of T cells. It is a member of the immunoglobulin superfamily containing two immunoglobulin C2-type domains. Both variants and alternative isoforms of CD158b have been reported. The interaction of CD158b with specific HLA-C antigens on a target cell (HLA-Cw1, HLA-Cw3, HLA-Cw7 alleles, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK cell and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The DX27 monoclonal antibody reacts with a common epitope of KIR2DL2 (CD158b1, p58.2), KIR2DL3 (CD158b2, p58.3), and KIR2DS2 (CD158j, p50.2). Additional reported applications (for the relevant formats) include: restoring the NK cell cytotoxicity1,5.This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bakker ABH, et al. 1998. J. Immunol. 160:5239. Lucas M, et al. 2003. J. Virol. 77:2251. Goodier M, et al. 2000. J. Immunol. 165:139. Yawata M, et al. 2002. Immunogenetics 54:543. Valiante NM, et al. 1997. Immunity 7:739.\u003cbr\u003e\nProduct Citations: Siepe DH, et al. 2022. Elife. 11:. PubMed Chen M, et al. 2021. Cancers (Basel). 13:. PubMed Ahmad F, et al. 2014. J Virol. 88:12397. PubMed Choi J, et al. 2018. Ann Surg Treat Res. 94:312. PubMed Bertulli C, et al. 2020. Int J Mol Sci. 21:. PubMed Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed Sitlinger A, et al. 2021. Cells. 10:. PubMed Grutza R, et al. 2020. J Immunol. 204:2910. PubMed Lubaki N, et al. 2016. PLoS Pathog. 12:e1006031. PubMed\u003cbr\u003e\nRRID: AB_2563376 (BioLegend Cat. No. 312611) AB_2563377 (BioLegend Cat. No. 312612)\u003cbr\u003e\nStructure: Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, type I membrane protein\u003cbr\u003e\nDistribution: NK cells and a subset of T cells\u003cbr\u003e\nFunction: Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-C antigens on target cell\u003cbr\u003e\nLigand\/Receptor: HLA-C antigens such as HLA-Cw1, HLA-Cw3, HLA-Cw7\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Colonna M, et al. 1995. Science 268:405. 2. Uhrburg M, et al. 1997. Immunity 7:753. 3. Wagtmann N, et al. 1995. Immunity 3:801. 4. Dohring C, et al. 1996. Immunogenetics 44:227. 5. Maenaka K, et al. 1999. Structure 7:391.\u003cbr\u003e\nGene ID: 3803\u003cbr\u003e\nUniProt: View information about CD158b\/j on UniProt.org\u003cbr\u003e\nClone: DX27\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD158b1 (KIR2DL2, p58.2), CD158b2 (KIR2DL3, p58.3), CD158j (KIR2DS2, p50.2), KIR-NKAT2, CD158b\/CD158j\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863060959401,"sku":"312612","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-312612","provider":"Iright","version":"1.0","type":"link"}