{"product_id":"biolegend-313522","title":"Biolegend, 313522, Pacific Blue™ anti-human\/mouse\/rat CD278 (ICOS) Antibody, 100μg","description":"\u003cp\u003eICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28\/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h\/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse T cell clone D10.G4.1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim) Yagi J, et al. 2003. J. Immunol. 171:783. (FC) Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim) Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Zander R, et al. 2022. Immunity. 55:475. PubMed Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed Westernberg L, et al. 2016. J Allergy Clin Immunol. 138:571-578. PubMed Cassotta A, et al. 2019. Nat Med. 25:1402. PubMed Hong J, et al. 2014. J Immunol. 193:797. PubMed DeFuria J, et al. 2013. Proc Natl Acad Sci U S A. 110:5133. PubMed Amodio D, et al. 2018. J Immunol. 200:538. PubMed Yamamoto T, et al. 2015. Sci Transl Med. 7: 298ra120. PubMed Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed Blackburn M, et al. 2015. J Immunol. 195: 3227 - 3236. PubMed Vargas-Inchaustegui D, et al. 2016. J Immunol. 196: 1700 - 1710. PubMed\u003cbr\u003e\nRRID: AB_10895762 (BioLegend Cat. No. 313521) AB_10899736 (BioLegend Cat. No. 313522)\u003cbr\u003e\nStructure: CD28\/CTLA-4, 47-57 kD\u003cbr\u003e\nDistribution: Activated T cells, subset of thymocytes\u003cbr\u003e\nFunction: Costimulates T cell activation, proliferation, humoral immune response\u003cbr\u003e\nLigand\/Receptor: B7h\/B7RP-1\/GL-50\u003cbr\u003e\nCell Type: T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff A, et al. 1999. Nature 397:263. 3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle AJ, et al. 2000. Immunity 13:95.\u003cbr\u003e\nGene ID: 1000488412985164545\u003cbr\u003e\nUniProt: View information about CD278 on UniProt.org\u003cbr\u003e\nClone: C398.4A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Inducible COStimulatory molecule, H4\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862943158441,"sku":"313522","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-313522","provider":"Iright","version":"1.0","type":"link"}