{"product_id":"biolegend-313712","title":"Biolegend, 313712, Alexa Fluor® 647 anti-human CD195 (CCR5) Antibody, 100tests","description":"\u003cp\u003eCD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. Recent studies have shown CCR5 to play a role in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO cells transfected with human CCR5\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm \/ 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1Clone HEK\/1\/85a and clone HEK\/1\/85a\/7a are the same.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mueller A, et al. 2002. Blood 99:785. Al-Odi E, et al. 2012. PLoS One. 7:e42217. PubMed Osman A, et al. 2014. J Immunol. 192:5083. PubMed Liu S, et al. 2014. Virus Res. 192:92. PubMed\u003cbr\u003e\nProduct Citations: Park A, et al. 2016. Mol Ther Methods Clin Dev. 3:16057. PubMed Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed Piryani SO, et al. 2019. Stem Cell Reports. 13:76. PubMed Mandl M, et al. 2014. PLoS One. 9:112140. PubMed Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed Achour L, et al. 2008. Blood. 101182. PubMed Tadagaki K, et al. 2012. Blood. 119:4908. PubMed Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed\u003cbr\u003e\nRRID: AB_528759 (BioLegend Cat. No. 313711) AB_528760 (BioLegend Cat. No. 313712)\u003cbr\u003e\nStructure: G-coupled receptor family 1, membrane protein, 45 kD\u003cbr\u003e\nDistribution: Subset of T cells, monocytes\u003cbr\u003e\nFunction: Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1\u003cbr\u003e\nLigand\/Receptor: MIP-1α, MIP-1ß, RANTES\u003cbr\u003e\nCell Type: Dendritic cells, Monocytes, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Samson M, et al. 1996. Biochemistry 35:3362. 2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161. 3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147. 4. Deng H, et al. 1996. Nature 381:661. 5. Lai J, et al. 2003. CVI. 10:1123. 6. Mañes S, et al. 2003. J. Exp. Med. 198:1381. 7. Vaday GG, et al. 2006. Prostate 66:124.\u003cbr\u003e\nGene ID: 1234\u003cbr\u003e\nUniProt: View information about CD195 on UniProt.org\u003cbr\u003e\nClone: HEK\/1\/85a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866455593129,"sku":"313712","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-313712","provider":"Iright","version":"1.0","type":"link"}