{"product_id":"biolegend-314501","title":"Biolegend, 314501, Purified anti-human IgM Antibody, 25μg","description":"\u003cp\u003eIgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ELISA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per 106 in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed\u003cbr\u003e\nProduct Citations: Mclaurin JD, et al. 2019. Mol Biol Cell. 30:1610. PubMed Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Pearson JA, et al. 2022. Diabetologia. 65:1398. PubMed Mann ER, et al. 2020. Sci Immunol. :5. PubMed Pyfrom SC, et al. 2020. J Immunol. 205:595. PubMed Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed Kolhatkar N, et al. 2015. J Exp Med. 212: 1663-1677. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed Georg P, et al. 2022. Cell. 185:493. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Waugh KA, et al. 2020. Cell Reports. 29(7):1893-1908.e4.. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed\u003cbr\u003e\nRRID: AB_493003 (BioLegend Cat. No. 314502)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 3507\u003cbr\u003e\nUniProt: View information about IgM on UniProt.org\u003cbr\u003e\nClone: MHM-88\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin M\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866132697257,"sku":"314501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-314501","provider":"Iright","version":"1.0","type":"link"}