{"product_id":"biolegend-314508","title":"Biolegend, 314508, PE anti-human IgM Antibody, 100tests","description":"\u003cp\u003eIgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed\u003cbr\u003e\nProduct Citations: Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed He WT, et al. 2022. Sci Transl Med. 14:eabl9605. PubMed Zhou P, et al. 2023. Immunity. 56:669. PubMed Starr TN, et al. 2021. Nature. 597:97. PubMed Tortorici MA, et al. 2021. Nature. 597:103. PubMed Tian L, et al. 2021. Int J Mol Sci. 22: . PubMed Yeung Y, et al. 2016. Nat Commun. 7:13376. PubMed Pontarini E, et al. 2015. Rheumatology. 54: 1429-1434. PubMed Biswas S, et al. 2021. Nature. 591:464. PubMed Shin JJ, et al. 2022. J Clin Immunol. :. PubMed Singh M, et al. 2020. Cell. 180(5):878-894. PubMed He WT, et al. 2022. Nat Immunol. 23:960. PubMed Graham C, et al. 2021. Immunity. 54(6):1276-1289.e6. PubMed Seow J, et al. 2022. Cell Rep. 39:110757. PubMed Inui M, et al. 2015. Int Immunol. 27: 345 - 355. PubMed Upton D, Unniraman S 2011. J Vis Exp. 57: 3573. PubMed Choi J, et al. 2020. Proc Natl Acad Sci U S A. 117:6092. PubMed Zhao F, et al. 2020. Cell Rep. 32:108122. PubMed Gonzales SJ, et al. 2022. Front Immunol. 13:809264. PubMed Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed\u003cbr\u003e\nRRID: AB_493006 (BioLegend Cat. No. 314507) AB_493005 (BioLegend Cat. No. 314508)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 3507\u003cbr\u003e\nUniProt: View information about IgM on UniProt.org\u003cbr\u003e\nClone: MHM-88\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin M\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866132304041,"sku":"314508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-314508","provider":"Iright","version":"1.0","type":"link"}