{"product_id":"biolegend-317354","title":"Biolegend, 317354, Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD3 SF Antibody, 1mg","description":"\u003cp\u003eCD3ε is a 20 kD chain of the CD3\/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α\/β or γ\/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:\u003cbr\u003e\n1mg\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: 0.1 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is \u0026lt; 0.01 EU\/µg of the protein (\u0026lt; 0.001 ng\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/μg of the protein (\u0026lt; 0.001 ng\/μg of the protein) as determined by the LAL test\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1.0 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: Activ FC Cell Culture\u003cbr\u003e\nRecommended Usage: 1 µg\/mL of antibody antibody in PBS, for plate coating protocols; 10 µg\/mL of OKT3 antibody for soluble antibody activation methods\u003cbr\u003e\nApplication Notes: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nAdditional Product Notes: This antibody is intended for activation and expansion of CD3 T cells in research and ex vivo cell culture applications. The antibody was GMP manufactured under serum-free conditions, including serum-free hybridoma cell culture, without additional animal or human-derived materials. OKT3 antibody can be used in combination with anti-human CD28 antibodies to efficiently activate T cells when used at 1 µg\/mL of antibody in PBS, for plate coating protocols. Plates should be coated for 2 hours in a tissue culture incubator, or overnight at 4°C. For soluble antibody activation protocols we recommend 10 µg\/mL of OKT3 antibody and 5 µg\/mL of anti-human CD28.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Li B, et al. 2005. Immunology 116:487. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed Alter G, et al. 2008. J. Virol. 82:9668. PubMed Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed Pinto JP, et al. 2010. Immunology. 130:217. PubMed Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed\u003cbr\u003e\nProduct Citations: Baharom F, et al. 2022. Cell. 4317:185. PubMed Chai H, et al. 2023. Nat Commun. 14:213. PubMed\u003cbr\u003e\nDisclaimer: BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.\u003cbr\u003e\nStructure: Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α\/β or γ\/δ) form the CD3\/TCR complex, 20 kD\u003cbr\u003e\nDistribution: Mature T and NK T cells, thymocyte differentiation\u003cbr\u003e\nFunction: Antigen recognition, signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen bound to MHC\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. Lanier L, et al. 1986. J. Immunol. 137:2501.\u003cbr\u003e\nGene ID: 916\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: OKT3\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: T3, CD3ε\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866555633833,"sku":"317354","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-317354","provider":"Iright","version":"1.0","type":"link"}