{"product_id":"biolegend-320007","title":"Biolegend, 320007, PE anti-mouse\/rat\/human FOXP3 Antibody, 25tests","description":"\u003cp\u003eFOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + \/CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in \"scurfy\" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: Cynomolgus, Rhesus, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full-length FOXP3 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nAdditional Product Notes: View more applications data for this product in our Scientific Poster Library.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Roncador G, et al. 2005 Eur. J. Immunol. 35:1681. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol Yang ZZ, et al. 2006. Blood 107:3639. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. Groh V, et al. 2006. Nature Immunology 7:755. Lewkowicz P, et al. 2006 J. Immunol. 177:7155. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023. Bamias G, et al. 2007. J. Immunol. 178:1809. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed Davidson TS, et al. 2007. J. Immunol. 178:4022. MacDonald K PA, et al. 2007. Blood doi:10.1182\/blood-2007-01-067249. Jaffar Z, et al. 2007. J. Immunol. 179:6193. Müller M, et al. 2007. J. Immunol. 179:2774. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381 Klunker S, et al. 2009. J. Exp Med. PubMed Haque A, et al. 2010. J. Immunol. 184:2583. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed\u003cbr\u003e\nProduct Citations: Barsheshet Y, et al. 2022. Int J Mol Sci. 23:. PubMed Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed Wu X, et al. 2023. iScience. 26:106559. PubMed Chamorro BM, et al. 2023. Vaccines (Basel). 11:. PubMed Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed Tian J, et al. 2021. Autophagy. 17:2586. PubMed Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed Mishra R, et al. 2022. J Transl Med. 20:323. PubMed Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed Zongyi Y, et al. 2017. PLoS One. 10.1371\/journal.pone.0189617. PubMed Casadesús AV, et al. 2020. Oncoimmunology. 9:1770565. PubMed Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed Levi J, et al. 2020. J Nucl Med. . PubMed Deng Z, et al. 2020. World J Gastrointest Oncol. 1091:12. PubMed Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed Momin N, et al. 2022. Nat Commun. 13:109. PubMed Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed Tian X, et al. 2022. J Cell Mol Med. 26:693. PubMed Su X, et al. 2022. J Transl Med. 20:378. PubMed Fang J, et al. 2014. J Neuroimmunol. 269:9. PubMed Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed Wang R, et al. 2022. Front Immunol. 13:947756. PubMed Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed Yang Z, et al. 2021. Onco Targets Ther. 14:4239. PubMed Zeng Q, et al. 2022. iScience. 25:105151. PubMed Huang J, et al. 2014. J Immunol. 192:1972. PubMed Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed He S, et al. 2021. Front Pharmacol. 12:743837. PubMed Wang S, et al. 2022. J Inflamm Res. 14:7107. PubMed Tanaka S, et al. 2016. Appl Envir Microbiol. 82: 2693 - 2699. PubMed Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed Chen KH, et al. 2019. J Neuropathol Exp Neurol. 78:65. PubMed Jia Y, et al. 2022. J Transl Med. 20:281. PubMed Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed Rana MN, et al. 2021. Front Pharmacol. 12:643215. PubMed\u003cbr\u003e\nRRID: AB_492981 (BioLegend Cat. No. 320007) AB_492980 (BioLegend Cat. No. 320008)\u003cbr\u003e\nStructure: Forkhead\/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains\u003cbr\u003e\nDistribution: Nuclear; expressed in T regulatory cells\u003cbr\u003e\nFunction: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis\u003cbr\u003e\nInteraction: Interacts with DNA\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Hori S, et al. 2003. Science 299:1057.\u003cbr\u003e\nRegulation: FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation\u003cbr\u003e\nGene ID: 2037131738250943\u003cbr\u003e\nUniProt: View information about FOXP3 on UniProt.org\u003cbr\u003e\nClone: 150D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003cbr\u003e\nQ: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?\u003cbr\u003e\nA: It is not recommended. It is best to use PBMCs for this testing.\u003cbr\u003e\nQ: Can FOXP3 be costained with cytokines?\u003cbr\u003e\nA: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860811436201,"sku":"320007","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-320007","provider":"Iright","version":"1.0","type":"link"}