{"product_id":"biolegend-320907","title":"Biolegend, 320907, APC anti-human MICA\/MICB Antibody, 25tests","description":"\u003cp\u003e6D4 antibody reacts with a common epitope of the human nonclassical MHC class I chain-related protein A (MICA) and B (MICB), also known as PERB11.1 and PERB11.2. The MIC gene is located in MHC class I region. MICA\/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate β2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN-α-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8 + α\/β T cells and γ\/δ T cells, as well as suppression of T cell proliferation. MICA\/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases. The 6D4 antibody is able to block MIC mediated cytotoxicity.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) applications include: immunohistochemistry2,3,5 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation7, and blocking2,3 of MIC mediated cytotoxicity. The Ultra-LEAF™ Purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 320919 \u0026amp; 320120).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Groh V, et al. 1999. Science 279:1737. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. Groh V, et al. 2001. Nature Immunol. 2:255. Li Z, et al. 2000. Immunogenetics 51:246. Park EJ, et al. 2003. J. Immunol. 171:4131. Jinushi M, et al. 2003. J. Immunol. 171:5423. Wu J, et al. 2003. J. Immunol. 170:4196.\u003cbr\u003e\nProduct Citations: Martínez-Sánchez MV, et al. 2021. Cancers (Basel). 13: . PubMed Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Tran TA, et al. 2023. Front Immunol. 13:1009484. PubMed Mo C, et al. 2012. J Biol Chem. 287:19242. PubMed Thomas M, et al. 2016. PLoS One. 11: 0158505. PubMed Wu X, et al. 2020. Cancers (Basel). 12:. PubMed Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed Wu X, et al. 2021. Front Immunol. 12:731767. PubMed Yao C, et al. 2018. Autophagy. 1.854861111. PubMed Okita R, et al. 2012. J Immunol. 188:2136. PubMed Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed\u003cbr\u003e\nRRID: AB_493196 (BioLegend Cat. No. 320907) AB_493195 (BioLegend Cat. No. 320908)\u003cbr\u003e\nStructure: 65-75 kD glycoprotein, MHC class I-like transmembrane molecules that do not associate ß2-microglobulin, highly polymorphic\u003cbr\u003e\nDistribution: Intestinal epithelium, epithelial tumors, endothelial cells, fibroblasts, INF-a-stimulated dendritic cells\u003cbr\u003e\nFunction: Activation of NK cells, subset of CD8+ α\/ß T cells and γ\/δ T cells, suppression of T cell proliferation\u003cbr\u003e\nLigand\/Receptor: NKG2D\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Groh V, et al. 1996. Proc. Natl. Acad. Sci. USA. 93:12445. 2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. 3. Jinushi M, et al. 2003. J. Immunol. 170:1249. 4. Kriegeskorte AK, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:11805. 5. Groh V, et al. 1999. Science 279:1737.\u003cbr\u003e\nGene ID: 1005074364277\u003cbr\u003e\nUniProt: View information about MICA MICB on UniProt.org\u003cbr\u003e\nClone: 6D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PERB11, MIC, MHC class I chain-related protein A, MICA, PERB11.1, MHC class I chain-related protein B, MICB, PERB11.2\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862475034793,"sku":"320907","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-320907","provider":"Iright","version":"1.0","type":"link"}