{"product_id":"biolegend-323503","title":"Biolegend, 323503, Biotin anti-human CD140a (PDGFRα) Antibody, 25μg","description":"\u003cp\u003eThe 16A1 monoclonal antibody recognizes human CD140a also known as the platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, and PDGFRα. CD140a is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140a contains three immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight of approximately 123 kD. CD140a is widely expressed on a variety of mesenchymal-derived cells and has been implicated in the development of some tumors including basal cell carcinoma and gastric stromal cell tumors. Binding of A-chain containing PDGF molecules as well as protease-activated PDGF-C molecules can stimulate cell proliferation. CD140a has been shown to interact with a number of proteins including CRK, Grb2, Grb14, SHP2, and others as integrin β3, caveolin-1, and nexin sorting molecules. The PDGFRα is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. The 16A1 antibody has been shown to be useful for flow cytometric detection of CD140a.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: NIH 3T3 cells transfected with human PDGFRalpha\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Miyazaki S et al. In:Leukocyte Typing VI Kishimoto et al. Eds, Garland Publishing Inc, New York 1998 pp 3-20. Lottaz C, et al. 2010. Cancer Res. 70:2030. PubMed Ricono JM, et al. 2009. Am. J. Physiol. Renal Physiol. 296:F406. (IF) Guarnerio J, et al. 2015. Cancer Discov. 5:396. PubMed\u003cbr\u003e\nProduct Citations: Niwa A, et al. 2011. PLoS One. 6:e22261. PubMed Evseenko D, et al. 2010. Proc Natl Acad Sci U S A. 107:13742. PubMed Ishimine H, et al. 2013. Biochem Biophys Res Commun. 438:753. PubMed\u003cbr\u003e\nRRID: AB_755997 (BioLegend Cat. No. 323503) AB_2162487 (BioLegend Cat. No. 323504)\u003cbr\u003e\nStructure: Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family.\u003cbr\u003e\nDistribution: Widely expressed on a variety of mesenchymal-derived cells.\u003cbr\u003e\nFunction: Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Knock-out studies have implicated an essential role for CD140a in kidney development. Has been implicated in basal cell carcinoma and gastric stromal cell tumors.\u003cbr\u003e\nInteraction: Interacts with Crk, as well as a variety of adaptor molecules and signaling intermediates (Grb2, Grb14, SHP2, others). Has also been shown to associate with integrin β3, caveolin-1, and nexin sorting molecules\u003cbr\u003e\nLigand\/Receptor: Binds to A-chain containing PDGF molecules and protease-activated PDGF-C molecules\u003cbr\u003e\nModification: Multiple tyrosine phosphorylation sites (Y720, Y731, Y742, Y754, Y762, Y767, Y768, Y988, Y993, Y1018)\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Gronwald RG, et al. 1988. Proc. Natl. Acad. Sci. USA 85:3435. 2. Gilbertson DG, et al. 2001. J. Biol. Chem. 276:27406. 3. Seifert RA, et al. 1989. J. Biol. Chem. 264:8771. 4. Rupp E, et al. 1994. Eur. J. Biochem. 225:29.\u003cbr\u003e\nGene ID: 5156\u003cbr\u003e\nUniProt: View information about CD140a on UniProt.org\u003cbr\u003e\nClone: 16A1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, PDGFRα, PDGFRa, PDGF receptor alpha\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860818677929,"sku":"323503","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-323503","provider":"Iright","version":"1.0","type":"link"}