{"product_id":"biolegend-324502","title":"Biolegend, 324502, Purified anti-human IgD Antibody, 100μg","description":"\u003cp\u003eIgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a\/CD79b (Igα\/Igβ) heterodimer, resulting in the activation of the B cell.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full length human IgD recombinant protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested SB - Community verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.\u003cbr\u003e\nRRID: AB_2888899 (BioLegend Cat. No. 324502)\u003cbr\u003e\nStructure: Exists in a transmembranal and a soluble form\u003cbr\u003e\nDistribution: Naïve B cells\u003cbr\u003e\nFunction: Antigen binding, B cell activation\u003cbr\u003e\nInteraction: The CD79a\/CD79b heterodimer\u003cbr\u003e\nMolecular Family: Immune Checkpoint Receptors, Innate Immune Signaling\u003cbr\u003e\nAntigen References: Geisberger R, et al. 2006. Immunology 118:429-37. Weller S, et al. 2005. Eur J Immunol. 35:2789-92. Brandtzaeg P \u0026amp; Johansen FE. 2005. Immunol Rev. 206:32-63.\u003cbr\u003e\nGene ID: 3495\u003cbr\u003e\nUniProt: View information about IgD on UniProt.org\u003cbr\u003e\nClone: W18340A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ig delta chain C region\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?\u003cbr\u003e\nA: It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.\u003cbr\u003e\nQ: Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?\u003cbr\u003e\nA: Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF\/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.\u003cbr\u003e\nQ: Are other fluorophores compatible with IBEX?\u003cbr\u003e\nA: Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.\u003cbr\u003e\nQ: The same antibody works in one tissue type but not another. What is happening?\u003cbr\u003e\nA: Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.\u003cbr\u003e\nQ: How can I be sure the staining I’m seeing in my tissue is real?\u003cbr\u003e\nA: In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866339168425,"sku":"324502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-324502","provider":"Iright","version":"1.0","type":"link"}