{"product_id":"biolegend-328102","title":"Biolegend, 328102, Purified anti-human CD90 (Thy1) Antibody, 100μg","description":"\u003cp\u003eCD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34 + cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34 + CD90 + cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HEL cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF® - Verified IHC-F, ICC, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP) Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity) Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope) Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)\u003cbr\u003e\nProduct Citations: Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed Stice SL, et al. 2022. Environ Health Perspect. 130:117002. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed Floy ME, et al. 2022. STAR Protoc. 3:101275. PubMed Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed Azari S, et al. 2021. MBio. 12:e0184921. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed Lin J, et al. 2019. J Immunol Res. 2019:6929286. PubMed Han L, et al. 2019. Haematologica. 10.3324\/haematol.2018.205534. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed Crawford LB, et al. 2021. J Virol. 95:. PubMed Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed\u003cbr\u003e\nRRID: AB_940390 (BioLegend Cat. No. 328101) AB_940393 (BioLegend Cat. No. 328102)\u003cbr\u003e\nStructure: 25-35 kD glycoprotein, Ig superfamily\u003cbr\u003e\nDistribution: Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines\u003cbr\u003e\nFunction: Regulate hematopoiesis and neural cell growth, cell adhesion\u003cbr\u003e\nLigand\/Receptor: β3 integrin, β2 integrin\u003cbr\u003e\nCell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. McKenzie JL, et al. 1981. J. Immunol. 126:843. 2. Avalos AM, et al. 2002. Biol. Res. 35:231. 3. Wetzel A, et al. 2004. J. Immunol. 172:3850.\u003cbr\u003e\nGene ID: 7070\u003cbr\u003e\nUniProt: View information about CD90 on UniProt.org\u003cbr\u003e\nClone: 5E10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: Thy-1, Thy1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864165470377,"sku":"328102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-328102","provider":"Iright","version":"1.0","type":"link"}