{"product_id":"biolegend-328702","title":"Biolegend, 328702, Purified anti-human CD148 Antibody, 100μg","description":"\u003cp\u003eCD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leucocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leucocytes. CD148 as a leucocyte activation marker and may be involved in the regulation of T cell activation. Leucocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and\/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: PHA-stimulated human PBMC\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tangye SG. 1998. J. Immunol. 161:3249. (FC) Tangye SG. 1998. J. Immunol. 161:3803. Tangye SG. 1998. J. Exp. Med. 188:1691. Autschbach F. 1999. Tissue Antigens 54:485. Lin J and Weiss A. 2003. J. Cell Biol. 162:673. (IF) Whiteford, JR., et al. 2011. Mol Cell Biol. 22:3609. PubMed.\u003cbr\u003e\nProduct Citations: Ali H, et al. 2015. PLoS One. e0122465:10. PubMed Nelson J, et al. 2015. FASEB J. 3085:29. PubMed Whiteford J, et al. 2011. Mol Biol Cell. 3.422916667. PubMed\u003cbr\u003e\nRRID: AB_2269146 (BioLegend Cat. No. 328702)\u003cbr\u003e\nDistribution: Monocytes, T cells, B cells, NK cells, epithelial cells, fibrocytes, melanocytes and Schwann cells\u003cbr\u003e\nCell Type: B cells, Epithelial cells, Fibroblasts, Monocytes, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nGene ID: 5796\u003cbr\u003e\nUniProt: View information about CD148 on UniProt.org\u003cbr\u003e\nClone: A3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: HPTP-eta, DEP-1, p260\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862522482857,"sku":"328702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-328702","provider":"Iright","version":"1.0","type":"link"}