{"product_id":"biolegend-329501","title":"Biolegend, 329501, Purified anti-human CD244 (2B4) Antibody, 25μg","description":"\u003cp\u003eCD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ\/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedWB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397. Chuang SS, et al. 2000. Immunology. 100:378. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed Rossi LE, et al. 2012. J Leukoc Biol. 91:321. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed\u003cbr\u003e\nProduct Citations: Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Zhuang X, et al. 2019. Cancer Immunol Res. 0.94375. PubMed Hoffmann S, et al. 2011. J Immunol. 186:2757. PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed Kim N, et al. 2020. Cancers (Basel). 12:. PubMed Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed\u003cbr\u003e\nRRID: AB_1279194 (BioLegend Cat. No. 329502)\u003cbr\u003e\nStructure: 38 kD type I transmembrane protein, CD2 family member, Ig superfamily\u003cbr\u003e\nDistribution: NK, T subset, monocytes, basophils, and eosinophils\u003cbr\u003e\nFunction: Activation and inhibition of NK cell function\u003cbr\u003e\nLigand\/Receptor: CD48\u003cbr\u003e\nCell Type: Basophils, Eosinophils, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Lee KM, et al. 2003. J. Immunol. 170:4881. 2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. 3. Messmer B, et al. 2006. J. Immunol. 176:4646. 4. Boles KS, et al. 2001. Immunol. Rev. 181:234.\u003cbr\u003e\nGene ID: 51744\u003cbr\u003e\nUniProt: View information about CD244 on UniProt.org\u003cbr\u003e\nClone: C1.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VII 70350\u003cbr\u003e\nOther Names: 2B4, SLAMF4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862938177705,"sku":"329501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-329501","provider":"Iright","version":"1.0","type":"link"}