{"product_id":"biolegend-329541","title":"Biolegend, 329541, TotalSeq™-D0189 anti-human CD244 (2B4) Antibody, 10μg","description":"\u003cp\u003eCD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ\/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.\u003cbr\u003e\n10μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA\u003cbr\u003e\nPreparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: PG - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein. To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions. Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.\u003cbr\u003e\nAdditional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com\/totalseq\/single-cell-dna The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation. View more applications data for this product in our Application Technical Notes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397. Chuang SS, et al. 2000. Immunology. 100:378. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed Rossi LE, et al. 2012. J Leukoc Biol. 91:321. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed\u003cbr\u003e\nRRID: AB_2894670 (BioLegend Cat. No. 329541)\u003cbr\u003e\nStructure: 38 kD type I transmembrane protein, CD2 family member, Ig superfamily\u003cbr\u003e\nDistribution: NK, T subset, monocytes, basophils, and eosinophils\u003cbr\u003e\nFunction: Activation and inhibition of NK cell function\u003cbr\u003e\nLigand\/Receptor: CD48\u003cbr\u003e\nCell Type: Basophils, Eosinophils, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Lee KM, et al. 2003. J. Immunol. 170:4881. 2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. 3. Messmer B, et al. 2006. J. Immunol. 176:4646. 4. Boles KS, et al. 2001. Immunol. Rev. 181:234.\u003cbr\u003e\nGene ID: 51744\u003cbr\u003e\nUniProt: View information about CD244 on UniProt.org\u003cbr\u003e\nClone: C1.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VII 70350\u003cbr\u003e\nOther Names: 2B4, SLAMF4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nBarcode Sequence: TCGCTTGGATGGTAG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862938407081,"sku":"329541","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-329541","provider":"Iright","version":"1.0","type":"link"}