{"product_id":"biolegend-334637","title":"Biolegend, 334637, Brilliant Violet 711™ anti-human FcεRIα Antibody, 25tests","description":"\u003cp\u003eHigh affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710\/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.4\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yamaguchi M, et al. 1999. J. Immunol. 162:5455. Suzukawa M, et al. 2005. Int. Immunol. 17:1249. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yamaguchi M, et al. 1999. J. Immunol. 162:5455.\u003cbr\u003e\nProduct Citations: Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed\u003cbr\u003e\nRRID: AB_2687185 (BioLegend Cat. No. 334637) AB_2687186 (BioLegend Cat. No. 334638)\u003cbr\u003e\nStructure: Ig superfamily, 50 kD\u003cbr\u003e\nDistribution: Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients\u003cbr\u003e\nFunction: Bind IgE, trigger IgE-mediated allergic response\u003cbr\u003e\nLigand\/Receptor: IgE\u003cbr\u003e\nCell Type: Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Fc Receptors\u003cbr\u003e\nAntigen References: 1. Riske F, et al. 1991. J. Biol. Chem. 266:11245 2. Gounni AS, et al. 2001. FASEB J. 15:940. 3. Maurer D, et al. 1996. J. Immunol. 157:607 4. Maurer d, et al. 1994. J. Exp. Med. 179:745 5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92.\u003cbr\u003e\nGene ID: 2205\u003cbr\u003e\nUniProt: View information about FcepsilonRIalpha on UniProt.org\u003cbr\u003e\nClone: AER-37 (CRA-1)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862533394601,"sku":"334637","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-334637","provider":"Iright","version":"1.0","type":"link"}