{"product_id":"biolegend-336307","title":"Biolegend, 336307, PE anti-human CD97 Antibody, 25tests","description":"\u003cp\u003eCD97 is a seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family with molecular weights of 74 kD, 80 kD, and 86 kD. It is expressed on monocytes\/macrophages, granulocytes, dendritic cells, and smooth muscle cells.The expression of CD97 on resting T- and B-lymphocytes is at low level, but is rapidly upregulated upon activation. CD97 binds to CD55 (decay-accelerating factor), chondroitin sulfate, integrin a5b1, and a3b1. CD97 has been shown to mediate cell adhesion and co-stimulation of T cell proliferation.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KG1a (human myeloid cell line)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone VIM3b bindswithin the first EGF domain of CD97.4 Additional reported applications include: western blot, immunoprecipitation.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hamann J, et al. 1995. J. Immunol. 155:1942. Lawrence DW, et al. 2003. J. Exp. Med. 198:999. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Wobus M, et al. 2004. Int. J. Cancer 112:815.\u003cbr\u003e\nProduct Citations: Nakajima H, et al. 2007. J Neurochem. 2428:103. PubMed de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed\u003cbr\u003e\nRRID: AB_1227612 (BioLegend Cat. No. 336307) AB_2076182 (BioLegend Cat. No. 336308)\u003cbr\u003e\nStructure: Seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family, 74 kD, 80 kD, 86 kD\u003cbr\u003e\nDistribution: Monocytes\/macrophages, granulocytes, dendritic cells, low levels on T cells and B-lymphocytes and upregulated upon activation\u003cbr\u003e\nFunction: Co-stimulation, adhesion\u003cbr\u003e\nLigand\/Receptor: CD55, chondroitin sulfate, integrin α5β1, integrin α3β1\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Capasso M, et al. 2006. J. Immunol. 177:1070 2. Wang T, et al. 2005. Blood 105:2836 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley \u0026amp; Sons Inc, Publication\u003cbr\u003e\nGene ID: 976\u003cbr\u003e\nUniProt: View information about CD97 on UniProt.org\u003cbr\u003e\nClone: VIM3b\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V BP030\u003cbr\u003e\nOther Names: BL-KDD\/F12\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864083124393,"sku":"336307","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-336307","provider":"Iright","version":"1.0","type":"link"}