{"product_id":"biolegend-337303","title":"Biolegend, 337303, FITC anti-human CD172a\/b (SIRPα\/β) Antibody, 25tests","description":"\u003cp\u003eSIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that belong to Ig superfamily. SIRPa (CD172a, SIRPb),(SIRPb1,CD172b), and SIRPg (SIRPb2,CD172g) have been described. CD172a\/b molecules are expressed on myeloid cells and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration. CD47 (IAP) is an extracellular ligand for CD172a. CD172b is a disulfide-linked homodimer. CD172b has been shown to regulate neutrophil transepithelial migration through the interaction with DAP12 and Syk kinase (through TYROBP).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications include: mAb 148 is specific for CD172a\/b, and also stains CD172g transfected cells, but not T-lymphocytes\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Piccio L, et al. 2005. Blood 105:2421 Dietrich J, et al. 2000. J. Immunol. 164:9\u003cbr\u003e\nProduct Citations: Connor SM, et al. 2022. Front Cell Neurosci. 16:894601. PubMed\u003cbr\u003e\nRRID: AB_2254620 (BioLegend Cat. No. 337304)\u003cbr\u003e\nStructure: Signal-regulatory-protein family, Ig superfamily\u003cbr\u003e\nDistribution: Monocytes\/macrophages, granulocytes, dendritic cells, neurons\u003cbr\u003e\nLigand\/Receptor: CD172a binds CD47, CD172b interacts with DAP12\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Kharitonenkov A, et al. 1997. Nature 386:181. 2. Tomasello E, et al. 2000. Eur. J. Immunol. 30:2147. 3. Seiffert M, et al. 2001. Blood 97:2741. 4. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838. 5. Barclay AN and MH. Brown. 2006. Nat. Rev.Immnuol. 6:457\u003cbr\u003e\nGene ID: 10326140885\u003cbr\u003e\nUniProt: View information about CD172a\/b on UniProt.org\u003cbr\u003e\nClone: 148\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SIRPa, SIRPb, SIRPalpha\/beta, BIT, SHPS1, MFR, P84, PTPNS1, CD172 antigen-like family member A, CD172 antigen-like family member B\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860805865641,"sku":"337303","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-337303","provider":"Iright","version":"1.0","type":"link"}