{"product_id":"biolegend-338810","title":"Biolegend, 338810, Brilliant Violet 421™ anti-human CD44 Antibody, 100tests","description":"\u003cp\u003eCD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Normal human PBL\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kishimoto T, et al. eds. 1997 Leucocyte Typing VI:White Cell Differentiation Antigen. Garland Publishing Inc.\u003cbr\u003e\nProduct Citations: Ender F, et al. 2017. PLoS One. 12(2):e0172446. PubMed Zhang J, et al. 2023. Nat Commun. 14:2439. PubMed Tyagi N, et al. 2016. Cancer Lett. 370: 260-267. PubMed Nelson L, et al. 2020. Nat Commun. 1.029166667. PubMed\u003cbr\u003e\nRRID: AB_2562405 (BioLegend Cat. No. 338809) AB_2562406 (BioLegend Cat. No. 338810)\u003cbr\u003e\nStructure: Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD\u003cbr\u003e\nDistribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells\u003cbr\u003e\nFunction: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM\u003cbr\u003e\nLigand\/Receptor: Hyaluronan, MIP-1β, fibronectin, collagen\u003cbr\u003e\nCell Type: Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Haynes BF, et al. 1991. Cancer Cells 3:347. 3. Goldstein LA, et al. 1989. Cell 56:1063. 4. Mikecz K, et al. 1995. Nat. Med. 1:558.\u003cbr\u003e\nGene ID: 960\u003cbr\u003e\nUniProt: View information about CD44 on UniProt.org\u003cbr\u003e\nClone: BJ18\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI A034\u003cbr\u003e\nOther Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862910029993,"sku":"338810","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-338810","provider":"Iright","version":"1.0","type":"link"}