{"product_id":"biolegend-339023","title":"Biolegend, 339023, Ultra-LEAF™ Purified anti-human CD122 (IL-2Rβ) Antibody, 100μg","description":"\u003cp\u003eCD122 is a 70-75 kD type I transmembrane glycoprotein and member of the Ig superfamily. It is IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. CD122 is constitutively expressed by NK cells and at lower levels by a subset of T cells. Its expression is upregulated upon activation. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: TL-Mor cell line\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/µg of the protein (\u0026lt; 0.001 ng\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. Older lots may have also been bottled at 1 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality testedFA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications include (for the relevant formats) include: immunoprecipitation, blocking of IL-2 binding to CD122, and partial inhibition of IL-2 induced cell proliferation.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takeshita T, et al. 1989. J. Exp. Med. 169:1323.\u003cbr\u003e\nRRID: AB_2860875 (BioLegend Cat. No. 339023) AB_2860876 (BioLegend Cat. No. 339024)\u003cbr\u003e\nStructure: Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD\u003cbr\u003e\nDistribution: NK cells, T subset\u003cbr\u003e\nFunction: Critical component of IL-2 and IL-15 signaling\u003cbr\u003e\nLigand\/Receptor: IL-2, IL-15\u003cbr\u003e\nCell Type: NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley \u0026amp; Sons Inc, Publication 2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472.\u003cbr\u003e\nGene ID: 3560\u003cbr\u003e\nUniProt: View information about CD122 on UniProt.org\u003cbr\u003e\nClone: TU27\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V C050\u003cbr\u003e\nOther Names: IL-2 Receptor β chain, IL-2Rβ\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864049111209,"sku":"339023","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-339023","provider":"Iright","version":"1.0","type":"link"}