{"product_id":"biolegend-341617","title":"Biolegend, 341617, Biotin anti-human CX3CR1 Antibody, 50μg","description":"\u003cp\u003eCX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes\/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CX3CR1-EGFP fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nishimura M, et al. 2002. J. Immunol. 168:6173. Nanki T, et al. 2002. Arthritis Rheum. 46:2878. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.\u003cbr\u003e\nProduct Citations: Giles JR, et al. 2022. Immunity. 55:557. PubMed\u003cbr\u003e\nRRID: AB_2616937 (BioLegend Cat. No. 341617)\u003cbr\u003e\nStructure: G-protein-coupled seven transmembrane receptor\u003cbr\u003e\nDistribution: NK cells, T cell subset, monocytes\/macrophages, dendritic cells\u003cbr\u003e\nFunction: cell adhesion and migration\u003cbr\u003e\nLigand\/Receptor: CX3CL1 (Fractalkine, FKN, neurotactin)\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Imai T, et al. 1997. Cell. 91:521. 2. Fong AM, et al. 1998. J. Exp. Med. 188:1413. 3. Auffray C, et al. 2009. J. Exp. Med. 206:595.\u003cbr\u003e\nGene ID: 1524\u003cbr\u003e\nUniProt: View information about CX3CR1 on UniProt.org\u003cbr\u003e\nClone: 2A9-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: V28, GPR13, Chemokine C-X3-C receptor 1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866539282601,"sku":"341617","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-341617","provider":"Iright","version":"1.0","type":"link"}