{"product_id":"biolegend-343921","title":"Biolegend, 343921, Brilliant Violet 421™ anti-human CD166 Antibody, 25tests","description":"\u003cp\u003eCD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Cultured human thymic epithelial cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pretzel D, et al. 2011. Arthritis Res. Ther. 13:R64. (IHC, IF, FC)\u003cbr\u003e\nRRID: AB_3083325 (BioLegend Cat. No. 343921) AB_3083325 (BioLegend Cat. No. 343922)\u003cbr\u003e\nStructure: A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins.\u003cbr\u003e\nDistribution: CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons.\u003cbr\u003e\nFunction: Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.\u003cbr\u003e\nLigand\/Receptor: CD6\u003cbr\u003e\nCell Type: Epithelial cells, Fibroblasts, Mesenchymal Stem Cells, Monocytes, Neurons, T cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Aruffo A, et al. 1997. Immunol Today. 18(10):498 2. Patel DD, et al. 1995. J. Exp. Med. 181:2213 3. Bowen MA, et al. 1995. J. Exp. Med. 181:1563 4. Horst D, et al. 2009. Cancer Invest. 22:1\u003cbr\u003e\nGene ID: 214\u003cbr\u003e\nUniProt: View information about CD166 on UniProt.org\u003cbr\u003e\nClone: 3A6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862386167977,"sku":"343921","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-343921","provider":"Iright","version":"1.0","type":"link"}