{"product_id":"biolegend-348501","title":"Biolegend, 348501, Purified anti-human CD144 (VE-Cadherin) Antibody, 25μg","description":"\u003cp\u003eCD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC - Verified WB, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.\u003cbr\u003e\nApplication Notes: Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.6 This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.7 Additional reported applications (for the relevant formats) include: Western Blotting1,2, immunofluorescence microscopy1,3, immunoprecipitation1,4, blocking angiogenesis in vitro4,5, inhibiting VE-cadherin reorganization4, and inducing endothelial cell apoptosis4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP) Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB) Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF) Corada M, et al. 2001. Blood 97:1679. (IP, Block) Kooistra M, et al. 2005. FEBS 579:4966. (Block) Corada M, et al. 2001. Blood 97:1679. (Block) Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.\u003cbr\u003e\nProduct Citations: Ghoroghi S, et al. 2021. Elife. 10:. PubMed Barzilai S, et al. 2017. Cell Rep. 18(3):685-699. PubMed Piao W, et al. 2020. Cell Reports. 30(4):1052-1062.e5.. PubMed Bashth OS, et al. 2020. Biomater Sci. 4.128472222. PubMed Boulanger MD, et al. 2022. PLoS One. 17:e0269316. PubMed Molina SG, et al. 2020. Stem Cell Res. 49:102015. PubMed Kondo N, et al. 2020. Cancers (Basel). 12:00. PubMed Liu X, et al. 2019. J Am Heart Assoc. 8:e013323. PubMed\u003cbr\u003e\nRRID: AB_10574464 (BioLegend Cat. No. 348501) AB_10588267 (BioLegend Cat. No. 348502)\u003cbr\u003e\nStructure: Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region\u003cbr\u003e\nDistribution: Endothelial cells, hematopoietic stem cells, perineurial cells\u003cbr\u003e\nFunction: Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis\u003cbr\u003e\nLigand\/Receptor: VE-cadherin\u003cbr\u003e\nCell Type: Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923. 2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223. 3. Kim I, et al. 2005. Blood 106:903. 4. Suzuki S, et al. 1991. Cell Regul. 2:261.\u003cbr\u003e\nGene ID: 1003\u003cbr\u003e\nUniProt: View information about CD144 on UniProt.org\u003cbr\u003e\nClone: BV9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: VE-Cadherin, cadherin-5, CDH5\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862928183465,"sku":"348501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-348501","provider":"Iright","version":"1.0","type":"link"}