{"product_id":"biolegend-350257","title":"Biolegend, 350257, StarBright UltraViolet 795 anti-human CD103 (Integrin αE) Antibody, 25tests","description":"\u003cp\u003eThis StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative .  This product is also available for direct purchase within a StarBright Basic Panel , which combines a minimal set of pre-selected compatible reagents. CD103 is a type I transmembrane glycoprotein also known as αE integrin, integrin αIEL chain, and human mucosal lymphocyte antigen 1. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. Treg cells express high level of CD103. Hairy cell leukemia has also been shown to express CD103. The expression of CD103 on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as an αE\/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Cynomolgus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HTLV-1 induced human T cell line MAPS16\u003cbr\u003e\nFormulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 795 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * StarBright UltraViolet 795 has a maximum excitation of 340 nm and a maximum emission of 792 nm. StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.\u003cbr\u003e\nExcitation Laser: Ultraviolet Laser (355 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western Blotting1, immunoprecipitation1, and immunohistochemical staining of frozen tissue sections1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636. (WB, IP, IHC-F) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, integrin family; can be cleaved into 150 kD and 25 kD chains; associated with β7 integrin\u003cbr\u003e\nDistribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells; expressed on hairy cell leukemia\u003cbr\u003e\nFunction: Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulation of tissue-specific T cell homing\u003cbr\u003e\nLigand\/Receptor: E-Cadherin\u003cbr\u003e\nCell Sources: Integrin β7\u003cbr\u003e\nCell Targets: Integrin β7\u003cbr\u003e\nCell Type: Dendritic cells, Lymphocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Parker CM, et al. 1992. P. Natl. Acad. Sci. USA 89:1924. 2. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636. 3. Schon MP, et al. 1999. J. Immunol. 162:6641. 4. Shaw SK, et al. 1994. J. Biol. Chem. 269:6016.\u003cbr\u003e\nGene ID: 3682\u003cbr\u003e\nUniProt: View information about CD103 on UniProt.org\u003cbr\u003e\nClone: Ber-ACT8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V A067\u003cbr\u003e\nOther Names: Integrin alpha E (ITGAE)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is a “StarBright panel”?\u003cbr\u003e\nA: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.\u003cbr\u003e\nQ: Will my StarBright panel be provided as a cocktail?\u003cbr\u003e\nA: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.\u003cbr\u003e\nQ: Are StarBright GMP\/ASR products available?\u003cbr\u003e\nA: StarBright ASR and GMP products are coming soon.\u003cbr\u003e\nQ: Are StarBright custom products available?\u003cbr\u003e\nA: Yes, please contact our Custom Solutions Team.\u003cbr\u003e\nQ: Will StarBright isotype controls be offered?\u003cbr\u003e\nA: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.\u003cbr\u003e\nQ: What amount of a StarBright conjugate should I use?\u003cbr\u003e\nA: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.\u003cbr\u003e\nQ: Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?\u003cbr\u003e\nA: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862908653737,"sku":"350257","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-350257","provider":"Iright","version":"1.0","type":"link"}