{"product_id":"biolegend-351310","title":"Biolegend, 351310, Brilliant Violet 421™ anti-human CD127 (IL-7Rα) Antibody, 100tests","description":"\u003cp\u003eCD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human CD127\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) application: proteogenomics1.\u003cbr\u003e\nAdditional Product Notes: View more applications data using this product to resolve dim populations, determine staining index, and to detect CLPs in our Scientific Poster Library.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)\u003cbr\u003e\nProduct Citations: Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed Anadon CM, et al. 2022. Cancer Cell. 40:545. PubMed Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed Trabanelli S, et al. 2017. Nat Commun. 10.1038\/s41467-017-00678-2. PubMed Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed Witkowski M, et al. 2021. Nature. 600:295. PubMed Wright AKA, et al. 2017. J Allergy Clin Immunol. 140:1430. PubMed de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed Ahmed R, et al. 2020. Cell Rep. 33:108501. PubMed Kim DH, et al. 2021. eLife. 10:00. PubMed Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed Tardif V, et al. 2019. Nat Commun. 10:823. PubMed Imai Y, et al. 2021. JID Innov. 1:100003. PubMed Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed Gyurdieva A, et al. 2022. Nat Commun. 13:5296. PubMed Morimoto Y, et al. 2018. Immunity. 49:134. PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed Platten M, et al. 2021. Nature. 592:463. PubMed Damele L, et al. 2021. Cancers (Basel). 13:. PubMed Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed Kimura K, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e210. PubMed Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed Xu Y, et al. 2013. J Immunol Methods. 394:73. PubMed Burke MT, et al. 2017. Transplant Direct. 3:e171. PubMed Xu Y, et al. 2013. J Virol. 87:3760. PubMed Willmann K, et al. 2014. Nat Commun. 5:5360. PubMed Crump NT, et al. 2021. Cell Reports. 35(6):109101. PubMed Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed Lev A, et al. 2019. Front Immunol. 1.577777778. PubMed Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed Zou F, et al. 2019. Nat Commun. 10:4109. PubMed O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed Vanoni G, et al. 2021. eLife. 10:00. PubMed Kasatskaya SA, et al. 2020. Elife. :9. PubMed Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed\u003cbr\u003e\nRRID: AB_10898326 (BioLegend Cat. No. 351309) AB_10960140 (BioLegend Cat. No. 351310)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD\u003cbr\u003e\nDistribution: Immature B cells through early pre-B stage, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells\u003cbr\u003e\nFunction: T cell and immature B cell proliferation and development\u003cbr\u003e\nLigand\/Receptor: IL-7\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.\u003cbr\u003e\nGene ID: 3575\u003cbr\u003e\nUniProt: View information about CD127 on UniProt.org\u003cbr\u003e\nClone: A019D5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-7 receptor α chain, IL-7Rα\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864383180969,"sku":"351310","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-351310","provider":"Iright","version":"1.0","type":"link"}