{"product_id":"biolegend-351318","title":"Biolegend, 351318, Alexa Fluor® 647 anti-human CD127 (IL-7Rα) Antibody, 100tests","description":"\u003cp\u003eCD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human CD127\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) application: proteogenomics1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)\u003cbr\u003e\nProduct Citations: Gusscott S, et al. 2016. PLoS One. 11: 0161158. PubMed Martin AR, et al. 2020. EBioMedicine. 62:103102. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Lenti MV, et al. 2022. Clin Transl Gastroenterol. 13:e00510. PubMed Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed Braza F, et al. 2015. J Am Soc Nephrol. 26: 1795-1805. PubMed Lima J, et al. 2016. Reprod Sci. 10.1177\/1933719116653680. PubMed Dehmani S, et al. 2021. Front Immunol. 12:732530. PubMed Dá\u0026amp;ncaron;ová K, et al. 2017. J Immunol. 198(2):729-740. PubMed\u003cbr\u003e\nRRID: AB_10900062 (BioLegend Cat. No. 351317) AB_10896063 (BioLegend Cat. No. 351318)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD\u003cbr\u003e\nDistribution: Immature B cells through early pre-B stage, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells\u003cbr\u003e\nFunction: T cell and immature B cell proliferation and development\u003cbr\u003e\nLigand\/Receptor: IL-7\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.\u003cbr\u003e\nGene ID: 3575\u003cbr\u003e\nUniProt: View information about CD127 on UniProt.org\u003cbr\u003e\nClone: A019D5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-7 receptor α chain, IL-7Rα\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864381051049,"sku":"351318","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-351318","provider":"Iright","version":"1.0","type":"link"}