{"product_id":"biolegend-351701","title":"Biolegend, 351701, Purified anti-human TCR Vα7.2 Antibody, 25μg","description":"\u003cp\u003eThe 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant TCR\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Martin E, et al. 2009. PLoS Biol. 7:525. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Shaler C, et al. 2017. Cancer Immunol Immunother. 10.1007\/s00262-017-2050-7. PubMed Souter MNT, et al. 2022. J Exp Med. 219:. PubMed Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed Wakao H, et al. 2013. Cell Stem Cell. 12:1. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Ye G, et al. 2021. Stem Cell Reports. 16:926. PubMed Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed Marrero I, et al. 2020. Alcohol Clin Exp Res. 44:1061. PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Jiang H, et al. 2022. Clin Transl Med. 12:e730. PubMed Nicol B,et al. 2017. J Autoimmun. . 10.1016\/j.jaut.2017.10.005. PubMed\u003cbr\u003e\nRRID: AB_10900258 (BioLegend Cat. No. 351702)\u003cbr\u003e\nStructure: Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans\u003cbr\u003e\nDistribution: Mucosal-associated invariant T (MAIT) cells and conventional T cells\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.\u003cbr\u003e\nGene ID: 28692\u003cbr\u003e\nUniProt: View information about TCR Valpha7.2 on UniProt.org\u003cbr\u003e\nClone: 3C10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor Vα7.2, TRAV1-2, T cell receptor alpha variable 1-2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862388822185,"sku":"351701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-351701","provider":"Iright","version":"1.0","type":"link"}