{"product_id":"biolegend-351707","title":"Biolegend, 351707, APC anti-human TCR Vα7.2 Antibody, 25tests","description":"\u003cp\u003eThe 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant TCR\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Martin E, et al. 2009. PLoS Biol. 7:525. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Yero A, et al. 2022. J Virol. 96:e0025522. PubMed Lejon K, et al. 2022. Scand J Immunol. 96:e13190. PubMed Wakao H, et al. 2013. Cell Stem Cell. 12:1. PubMed Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed Sattler A, et al. 2019. J Immunol. 203:2970. PubMed Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed Ben Youssef G, et al. 2018. J Exp Med. 215:459. PubMed Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed Kang S, et al. 2016. PLoS One. 10: 0004832. PubMed Dias J, et al. 2016. J Leukoc Biol. 100: 233 - 240. PubMed Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed McWilliam H, et al. 2016. Nat Immunol. 17: 531-537. PubMed Li H, et al. 2022. Cell Death Dis. 13:849. PubMed Keller A, et al. 2017. Nat Immunol. 18:402-411. PubMed Gracey E, et al. 2016. Ann Rheum Dis. 75(12):2124-2132. PubMed Valestrand L, et al. 2022. Am J Pathol. . PubMed Leng T, et al. 2019. Cell Rep. 28:3077. PubMed Wilgenburg B, et al. 2016. Nat Commun. 7:11653. PubMed Ling L, et al. 2016. Sci Rep. 6:20358. PubMed Cosgrove C, et al. 2013. Blood. 121:951. PubMed Cho Y, et al. 2014. J Immunol. 193:3891. PubMed\u003cbr\u003e\nRRID: AB_10933430 (BioLegend Cat. No. 351707) AB_10933246 (BioLegend Cat. No. 351708)\u003cbr\u003e\nStructure: Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans\u003cbr\u003e\nDistribution: Mucosal-associated invariant T (MAIT) cells and conventional T cells\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.\u003cbr\u003e\nGene ID: 28692\u003cbr\u003e\nUniProt: View information about TCR Valpha7.2 on UniProt.org\u003cbr\u003e\nClone: 3C10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor Vα7.2, TRAV1-2, T cell receptor alpha variable 1-2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862387445929,"sku":"351707","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-351707","provider":"Iright","version":"1.0","type":"link"}