{"product_id":"biolegend-353702","title":"Biolegend, 353702, Purified anti-human CD183 (CXCR3) Antibody, 100μg","description":"\u003cp\u003eHuman CXCR3, also known as GPR9, is a chemokine receptor that binds CXCL9, CXCL10, and CXCL11. It is a 38 kD seven-pass transmembrane receptor coupled to G-protein. CXCR3 is highly expressed by T cells (Th1), natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 is important for effector lymphocyte recruitment into inflamed tissue in various inflammatory and autoimmune diseases, such as chronically inflamed liver, Crohn's disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CXCR3 transfectants\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Spoerl S, et al. 2021. Eur J Immunol. 51:1436. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Kikuchi N, et al. 2019. Biol Pharm Bull. 42:57. PubMed Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed\u003cbr\u003e\nRRID: AB_10983073 (BioLegend Cat. No. 353702)\u003cbr\u003e\nStructure: CXC-chemokine receptor, G protein-coupled receptor, seven-pass transmembrane receptor\u003cbr\u003e\nDistribution: T cell subset, NK cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors, mast cells, alveolar macrophages, eosinophils, and airway epithelial cells\u003cbr\u003e\nFunction: Essential in T cell recruitment to sites of inflammation\u003cbr\u003e\nLigand\/Receptor: CXCL9, CXCL10, and CXCL11\u003cbr\u003e\nCell Type: Dendritic cells, Eosinophils, Epithelial cells, Hematopoietic stem and progenitors, Macrophages, Mast cells, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Loetscher M, et al. 1996. J. Exp. Med. 184:963. 2. Cole KE, et al. 1998. J. Exp. Med. 187:2009. 3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909. 4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887. 5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089. 6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67.\u003cbr\u003e\nGene ID: 2833\u003cbr\u003e\nUniProt: View information about CD183 on UniProt.org\u003cbr\u003e\nClone: G025H7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866093047977,"sku":"353702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-353702","provider":"Iright","version":"1.0","type":"link"}