{"product_id":"biolegend-355405","title":"Biolegend, 355405, APC anti-human CD369 (Dectin-1\/CLEC7A) Antibody, 25tests","description":"\u003cp\u003eCD369 (Dectin-1), also known as CLEC7A and beta-glucan receptor (BGR), is a 33 kD type II transmembrane C-type lectin. It consists of a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a short N-terminal ITAM containing a cytoplasmic tail. CLEC7A is predominantly expressed on dendritic cells. Subsequent work has shown expression on B cells, monocytes, eosinophils, mast cells, macrophages, endothelial cells, and low expression on some subsets of T cells. CLEC7A lacks residues involved in calcium ligation that mediates carbohydrate-binding by classical C-type lectins. CLEC7A functions as a receptor to mediate innate immunity to fungal pathogens by selectively binding beta-glucan polymers, a major component of yeast and mycobacterial cell walls.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Ectodomain with C-term hFc fusion, RIBLS.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The epitope of clone 15E2 is LWEDGSTFSSN. Clone 15E2 can recognize the epitope in the presence of Curdlan and Laminarin (CLEC7A ligands). Additional reported applications (for the relevant formats) include: immunohistochemical staining.1\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ni L, et al. 2010. J. Immunol. 185:3504. (IHC)\u003cbr\u003e\nRRID: AB_2800956 (BioLegend Cat. No. 355405) AB_2800957 (BioLegend Cat. No. 355406)\u003cbr\u003e\nStructure: Type II transmemberane C-type lectin, one carbohydrate recognition domain, ITAM containing cytoplasmic tail\u003cbr\u003e\nDistribution: B cells, low level of some subsets of T cells, monocytes, dendritic cells, macrophages and endothelial cells\u003cbr\u003e\nFunction: Mediates innate immunity to fungal pathogens\u003cbr\u003e\nInteraction: TLR2, CD37\u003cbr\u003e\nLigand\/Receptor: Beta-glucan\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Macrophages, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Goodridge HS, et al. 2011. Nature 472:471. 2. Saijo S, et al. 2007. Nat. Immunol. 8:39. 3. Brown GD. 2006. Nat. Rev. Immunol. 6:33. 4. Willment JA, et al. 2001. J. Biol. Chem. 276:43818. 5. Yokota K, et al. 2001. Gene 272:51.\u003cbr\u003e\nGene ID: 64581\u003cbr\u003e\nUniProt: View information about CD369 on UniProt.org\u003cbr\u003e\nClone: 15E2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: CLECSF12, beta-glucan receptor (BGR, betaGR), CD369\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860812583081,"sku":"355405","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-355405","provider":"Iright","version":"1.0","type":"link"}