{"product_id":"biolegend-356913","title":"Biolegend, 356913, FITC anti-human CD185 (CXCR5) Antibody, 25tests","description":"\u003cp\u003eCD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nProduct Citations: Biasci D, et al. 2020. Proc Natl Acad Sci U S A. 117:28960. PubMed Karim F, et al. 2021. Elife. 10:. PubMed Weiskopf D, et al. 2015. Proc Natl Acad Sci U S A. 112: E4256-E4263. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Amodio D, et al. 2018. J Immunol. 200:538. PubMed\u003cbr\u003e\nRRID: AB_2561895 (BioLegend Cat. No. 356913) AB_2561896 (BioLegend Cat. No. 356914)\u003cbr\u003e\nStructure: G-protein coupled receptor, 7 transmembrane regions, 42 kD\u003cbr\u003e\nDistribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.\u003cbr\u003e\nFunction: Mediates cell migration to the B cell follicles in the secondary lymphoid organs\u003cbr\u003e\nLigand\/Receptor: CXCL13\u003cbr\u003e\nCell Type: B cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241. 2. León B, et al. 2012. Nat. Immunol. 13:681. 3. Crotty S. 2011. Annu. Rev. Immunol. 29:621. 4. Kerfoot SM, et al. 2011. Immunity 34:947. 5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.\u003cbr\u003e\nGene ID: 643\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: J252D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, MDR15\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866111692969,"sku":"356913","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-356913","provider":"Iright","version":"1.0","type":"link"}