{"product_id":"biolegend-357103","title":"Biolegend, 357103, FITC anti-human CD77 Antibody, 25tests","description":"\u003cp\u003eCD77 is also known as Gb3 glycosphingolipid. It was found specifically expressed on Burkitt's lymphoma cells and on a subset of germinal center B lymphocytes. CD77 has been described as a receptor for Shiga Toxin and Verotoxin 1 (VT1), which is produced by Shigella dysenteriae and some strains of E. coli . It is used as a marker for germinal center stage B-cells. CD19 has been reported as a ligand for CD77 and their interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human peripheral blood mononuclear cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats) include: Western blotting1 and immunohistochemical staining of frozen tissue sections1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fyfe G, et al. 1987. J. Immunol. 139:2187. (WB, IHC)\u003cbr\u003e\nProduct Citations: Maldonado-Báez L, et al. 2013. J Cell Biol. 233:201. PubMed Mohamed LA, et al. 2019. Exp Neurol. 27:316. PubMed Roodsant TJ, et al. 2024. iScience. 109178:27. PubMed Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed\u003cbr\u003e\nRRID: AB_2562160 (BioLegend Cat. No. 357103) AB_2562161 (BioLegend Cat. No. 357104)\u003cbr\u003e\nStructure: Glycosphingolipid\u003cbr\u003e\nDistribution: Burkitt's lymphoma cells, a subset of germinal center B lymphocytes\u003cbr\u003e\nFunction: Involved in cell adhesion and signaling transduction by binding CD19\u003cbr\u003e\nLigand\/Receptor: Receptor of Shiga Toxin and Verotoxin 1 (VT1) and CD19\u003cbr\u003e\nCell Type: B cells, Lymphocytes\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Adhesion, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Tétaud C, 2003. J. Biol. Chem. 278:45200. 2. Maloney MD, 1994. J. Exp. Med. 1994 180:191. 3. Mangeney M, et al. 1991. Eur. J. Immunol. 21:1131. 4. Mangeney M, et al. 1993. Cancer Res. 53:5314.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: 5B5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI CD77.5\u003cbr\u003e\nOther Names: globotriaosylcermamide (Gb3), Burkitt's lymphoma associated antigen (BLA), cermadie trihexoside (CTH), and Pk blood group antigen\u003cbr\u003e\nIsotype: Mouse IgM, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862448099497,"sku":"357103","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-357103","provider":"Iright","version":"1.0","type":"link"}