{"product_id":"biolegend-360723","title":"Biolegend, 360723, Brilliant Violet 421™ anti-human CD16 Antibody, 25tests","description":"\u003cp\u003eCD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: NK cell-enriched fraction from human peripheral blood.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G84. Donor variability has been observed for clone B73.1 staining1, especially on granulocytes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Perussia B, et al. 1983. J. Immunol. 130:2133. Lanier LL, et al. 1985. J. Exp. Med. 162:2089. Perussia B, et al. 1984. J. Immunol. 133:180. Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)\u003cbr\u003e\nProduct Citations: Rodriguez Benavente MC, et al. 2023. Immunology. 202:170. PubMed Benavente MCR, et al. 2024. Sci Rep. 7938:14. PubMed Sullivan KD et al. 2016. eLife. 5 pii: e16220. PubMed\u003cbr\u003e\nRRID: AB_2616913 (BioLegend Cat. No. 360723) AB_2616914 (BioLegend Cat. No. 360724)\u003cbr\u003e\nStructure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)\u003cbr\u003e\nDistribution: NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells\u003cbr\u003e\nFunction: Low affinity IgG Fc receptor, phagocytosis, ADCC\u003cbr\u003e\nLigand\/Receptor: IgG Fc receptor III (FcγRIII)\u003cbr\u003e\nCell Type: Macrophages, Monocytes, Neutrophils, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: 1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711. 2. Palmer BE, et al. 2005. J. Immunol. 175:8415. 3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183. 4. Wood KL, et al. 2005. Clin. Immunol. 117:294. 5. Björkström NK, et al. 2008. J. Immunol. 181:4219.\u003cbr\u003e\nGene ID: 22142215\u003cbr\u003e\nUniProt: View information about CD16 on UniProt.org\u003cbr\u003e\nClone: B73.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3, CD16a\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862896038057,"sku":"360723","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-360723","provider":"Iright","version":"1.0","type":"link"}