{"product_id":"biolegend-360740","title":"Biolegend, 360740, PE anti-human CD16 Recombinant Antibody, 100tests","description":"\u003cp\u003eCD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nRRID: AB_3683382 (BioLegend Cat. No. 360739) AB_3683382 (BioLegend Cat. No. 360740)\u003cbr\u003e\nStructure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)\u003cbr\u003e\nDistribution: NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells\u003cbr\u003e\nFunction: Low affinity IgG Fc receptor, phagocytosis, ADCC\u003cbr\u003e\nInteraction: CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC)\u003cbr\u003e\nLigand\/Receptor: IgG Fc receptor III (FcγRIII)\u003cbr\u003e\nCell Type: Macrophages, Monocytes, Neutrophils, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: Rataj F, et al. 2019. Br J Cancer. 120:79-87. Kaufmann M, et al. 2021. Med. 2:296-312. Sigal N, et al. 2022. Methods Mol Biol. 2543:113-128. Cheung P, et al. 2018. Cell. 173:1385. Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. Roussel M, et al. 2021. Cell Reports Medicine. 2:100291. Waugh KA, et al. 2020. Cell Reports. 29:1893-1908.e4.\u003cbr\u003e\nGene ID: 22142215\u003cbr\u003e\nUniProt: View information about CD16 on UniProt.org\u003cbr\u003e\nClone: B73.1.Rec\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3, CD16a\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862896529577,"sku":"360740","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-360740","provider":"Iright","version":"1.0","type":"link"}