{"product_id":"biolegend-360904","title":"Biolegend, 360904, PE anti-human IL-27\/IL-35 EBI3 (monomer, dimer, heterodimer) Antibody, 100tests","description":"\u003cp\u003eEpstein-Barr virus induced gene-3, also known as EBI3, is an IL-12 p40-related protein that can heterodimerize with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 diferentiation, increase cytolytic potential of CD8+ T cells, and impact B cell differentiation and Ig production.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified human IL-27\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Alenazy MF, et al. 2021. Sci Rep. 11:5629. PubMed Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed Wang K, et al. 2018. Medicine (Baltimore). 97:e0710. PubMed\u003cbr\u003e\nRRID: AB_2562879 (BioLegend Cat. No. 360903) AB_2562880 (BioLegend Cat. No. 360904)\u003cbr\u003e\nStructure: Hematopoietin receptor family, subunit of IL-27 and IL-35\u003cbr\u003e\nDistribution: B cells, monocytes and dendritic cells\u003cbr\u003e\nFunction: Pro- and anti-inflammatory\u003cbr\u003e\nInteraction: IL-27 p28 subunit, IL-35 p35 subunit\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Pflanz S, et al. 2002. Immunity. 16: 779. 2. Takeda A, et al. 2003. J. immunol. 170: 4886. 3. Pflanz S, et al. 2004. J. Immunol. 172: 2225. 4. Li MF, et al. 2013. Dev. Comp. Immunol. 41: 514.\u003cbr\u003e\nGene ID: 10148\u003cbr\u003e\nUniProt: View information about IL-27 IL-35 on UniProt.org\u003cbr\u003e\nClone: B032F6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Epstein Barr virus induced gene 3, IL-27B, IL-35 subunit\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866080432297,"sku":"360904","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-360904","provider":"Iright","version":"1.0","type":"link"}