{"product_id":"biolegend-361901","title":"Biolegend, 361901, Purified anti-human CD45 Antibody, 25μg","description":"\u003cp\u003eCD45 is a 180-220 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Rhesus, Cynomolgus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human PBL\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fry T, et al. 2003. Blood 101:2294. Mabaera R, et al. 2008. Blood 111:411. Knapp W, et al. 1989. Leukocyte Typing IV. New York: Oxford University Press.\u003cbr\u003e\nProduct Citations: Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed\u003cbr\u003e\nRRID: AB_2563176 (BioLegend Cat. No. 361901) AB_2563177 (BioLegend Cat. No. 361902)\u003cbr\u003e\nStructure: Tyrosine phosphatases, type I transmembrane protein, 180-220 kD\u003cbr\u003e\nDistribution: Hematopoietic cells, not circulating erythrocytes or platelets\u003cbr\u003e\nFunction: TCR and BCR mediated activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nCell Type: B cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.\u003cbr\u003e\nGene ID: 5788\u003cbr\u003e\nUniProt: View information about CD45 on UniProt.org\u003cbr\u003e\nClone: Tü116\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: IV N832\u003cbr\u003e\nOther Names: LCA, T200\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864063463593,"sku":"361901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-361901","provider":"Iright","version":"1.0","type":"link"}