{"product_id":"biolegend-362102","title":"Biolegend, 362102, Purified anti-human CCX-CKR (CCRL1) Antibody, 100μg","description":"\u003cp\u003eHuman CCX-CKR, also known as CCRL1, is a 40 kD, G-protein-coupled, seven transmembrane receptor. CCRL1 is a decoy receptor for CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25 that regulates chemotaxis of lymphocytes and cancer cells. Chemokine binding by CCX-CKR causes its internalization, reducing levels of available chemokine, and thus regulating migration of lymphocytes and cancerous cells. CCX-CKR is expressed by stromal cells of skin-draining lymph nodes, thymic epithelial cells, and immature dendritic cells (DCs). A low CCX-CKR expression in tumor cells correlates with a poor survival rate of breast cancer patients.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length CCX-CKR cDNA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC, IP, Agonist - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: immunoprecipitation of CCX-CKR from cell lysates1, induction of CCX-CKR internalization1 and immunofluorescence1. 13E11 recognizes an epitope located within the first 14 amino acids of CCX-CKR.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takatsuka, S. et al. 2011. J. Pharmacol. Toxicol. Methods. 63:250. (IF, IP, Agonist)\u003cbr\u003e\nProduct Citations: Bryce S, et al. 2016. J Immunol. 196: 3341-3353. PubMed Matti C, et al. 2020. Front Immunol. 11:720. PubMed\u003cbr\u003e\nRRID: AB_2563201 (BioLegend Cat. No. 362102)\u003cbr\u003e\nStructure: G-protein-coupled seven transmembrane receptor, 40 kD.\u003cbr\u003e\nDistribution: Stromal cells of skin-draining lymph nodes, thymic epithelial cells, immature DCs.\u003cbr\u003e\nFunction: C-C type chemokine scavenger and decoy receptor, reduces chemokine availability by sequestering them inside the cell.\u003cbr\u003e\nInteraction: β-arrestin.\u003cbr\u003e\nLigand\/Receptor: CCL2, CCL8, CCL13, CCL19, CCL21, and CCL25.\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Watts AO, et al. 2013. J. Biol. Chem. 288:7169. 2. Comerford I, et al. 2010. Blood. 116:4130.\u003cbr\u003e\nGene ID: 51554\u003cbr\u003e\nUniProt: View information about CCX-CKR on UniProt.org\u003cbr\u003e\nClone: 13E11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ACKR4, CC-CKR-11, VSHK1, PPR1, CCBP2, CCR11, CKR-11, CCXCKR\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860801605801,"sku":"362102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-362102","provider":"Iright","version":"1.0","type":"link"}