{"product_id":"biolegend-364102","title":"Biolegend, 364102, Purified anti-BrdU Antibody, 100μg","description":"\u003cp\u003eBrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.\u003cbr\u003e\n100μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Iodouridine-conjugated ovalbumin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. Hirota K, et al. 2007. J. Exp. Med. 204:41. Godebu E, et al. 2008. J. Immunol. 181:1798. Waskow C, et al. 2008. Nat. Immunol. 9:676.\u003cbr\u003e\nProduct Citations: Pernet E, et al. 2023. Nature. 614:530. PubMed Shao F, et al. 2021. Nat Commun. 12:6540. PubMed Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed Sakurai Y, et al. 2018. Mol Ther Oncolytics. 11:102. PubMed\u003cbr\u003e\nRRID: AB_2564497 (BioLegend Cat. No. 364101) AB_2564498 (BioLegend Cat. No. 364102)\u003cbr\u003e\nStructure: Uridine derivative that can be incorporated into DNA and substitute thymidine residues.\u003cbr\u003e\nDistribution: Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.\u003cbr\u003e\nFunction: The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Immunology\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Barker JM, et al. 2013. PLoS One 8:e63692. 2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205. 3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855. 4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954. 5. van der Wath RC, et al. 2009. PLoS One 4:e6972. 6. Dolbeare F, et al. 1985. Cytometry 6:521. 7. Gratzner HG. 1982. Science 218:474.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: 3D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 5-bromodeoxyuridine, bromodeoxyuridine\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862393049257,"sku":"364102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-364102","provider":"Iright","version":"1.0","type":"link"}