{"product_id":"biolegend-364111","title":"Biolegend, 364111, Alexa Fluor® 700 anti-BrdU Antibody, 25tests","description":"\u003cp\u003eBrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.\u003cbr\u003e\n25tests\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Iodouridine-conjugated ovalbumin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm \/ 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. Hirota K, et al. 2007. J. Exp. Med. 204:41. Godebu E, et al. 2008. J. Immunol. 181:1798. Waskow C, et al. 2008. Nat. Immunol. 9:676.\u003cbr\u003e\nRRID: AB_2814312 (BioLegend Cat. No. 364111) AB_2814313 (BioLegend Cat. No. 364112)\u003cbr\u003e\nStructure: Uridine derivative that can be incorporated into DNA and substitute thymidine residues.\u003cbr\u003e\nDistribution: Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.\u003cbr\u003e\nFunction: The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Immunology\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Barker JM, et al. 2013. PLoS One 8:e63692. 2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205. 3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855. 4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954. 5. van der Wath RC, et al. 2009. PLoS One 4:e6972. 6. Dolbeare F, et al. 1985. Cytometry 6:521. 7. Gratzner HG. 1982. Science 218:474.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: 3D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 5-bromodeoxyuridine, bromodeoxyuridine\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862392721577,"sku":"364111","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-364111","provider":"Iright","version":"1.0","type":"link"}